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ProductsBack/SERPINF2 / Alpha-2-Antiplasmin Antibody

SERPINF2 / Alpha-2-Antiplasmin Antibody

LS-C147208
LifeSpan BioSciences
ApplicationsImmunoDiffusion, ImmunoElectrophoresis
Product group Antibodies
TargetSERPINF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SERPINF2 / Alpha-2-Antiplasmin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    This product is intended for use in precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). Detection of plasmin-antiplasmin complexes in plasma is evident of plasmin formation and fibrinolysis.. ID, IE This product is intended for use in precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). Detection of plasmin-antiplasmin complexes in plasma is evident of plasmin formation and fibrinolysis.
  • Applications
    ImmunoDiffusion, ImmunoElectrophoresis
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5345
  • Target name
    SERPINF2
  • Target description
    serpin family F member 2
  • Target synonyms
    A2AP; AAP; alpha-2-antiplasmin; alpha2AP; alpha-2-AP; ALPHA-2-PI; alpha-2-plasmin inhibitor; API; plasmin inhibitor; PLI; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
  • Host
    Goat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 2 Antiplasmin/SERPINF2 antigen affinity purified polyclonal antibody (Catalog # A04248-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 2 Antiplasmin/SERPINF2 at approximately 65 kDa. The expected band size for Alpha 2 Antiplasmin/SERPINF2 is at 65 kDa.
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