WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.
WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.
SETDB1 Rabbit Polyclonal Antibody
TA319369
Overview
- SupplierOriGene
- Product NameSETDB1 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID9869
- Target nameSETDB1
- Target descriptionSET domain bifurcated histone lysine methyltransferase 1
- Target synonymsERG-associated protein with a SET domain, ESET; ESET; H3-K9-HMTase4; histone H3-K9 methyltransferase 4; histone-lysine N-methyltransferase SETDB1; histone-lysine N-methyltransferase, H3lysine-9 specific 4; KG1T; KMT1E; lysine N-methyltransferase 1E; SET domain bifurcated 1; TDRD21; tudor domain containing 21
- HostRabbit
- IsotypeIgG
- Protein IDQ15047
- Protein NameHistone-lysine N-methyltransferase SETDB1
- Scientific DescriptionRabbit polyclonal anti-ESET antibody
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203