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ProductsBack/Sheep anti Human Glypican-3 (GPC3)

Sheep anti Human Glypican-3 (GPC3)

X2395P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGPC3
500 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human Glypican-3 (GPC3)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for immunohistochemistry on paraffin-embedded tissues (10 microg/ml) and Western blotting (5-10 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (paraffin);Western Blotting;Immunohistochemistry (paraffin)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2719
  • Target name
    GPC3
  • Target description
    glypican 3
  • Target synonyms
    DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican-3, glypican proteoglycan 3, heparan sulphate proteoglycan, intestinal protein OCI-5, secreted glypican-3
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDP51654
  • Protein Name
    Glypican-3
  • Scientific Description
    GPC3, GPC-3, OCI5, OCI-5, GTR2-2, MXR7
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    C terminal recombinant protein (~70aa) which does not overlap with clone 1G12 epitope)
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
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