ICC/IF analysis of PFA-fixed HeLa cells using GTX00951 SIRT1 antibody [GT1189]. Red : Primary antibody Blue : DAPI
![WB analysis of wild-type (WT) and SIRT1 knockout (KO) HeLa cell extracts using GTX00951 SIRT1 antibody [GT1189]. Dilution : 1:1000 Laoding : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00951/GTX00951_20200327_WB_1_w_23053121_898.webp "WB analysis of wild-type (WT) and SIRT1 knockout (KO) HeLa cell extracts using GTX00951 SIRT1 antibody [GT1189]. Dilution : 1:1000 Laoding : 25microg")
![WB analysis of various samples using GTX00951 SIRT1 antibody [GT1189]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00951/GTX00951_20200327_WB_2_w_23053121_435.webp "WB analysis of various samples using GTX00951 SIRT1 antibody [GT1189]. Dilution : 1:1000 Loading : 25microg")
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody [GT1189] (GTX00951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00951/GTX00951_4000000146_20200313_WB_TPM_watermark_w_23053121_229.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody [GT1189] (GTX00951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
ICC/IF analysis of PFA-fixed HeLa cells using GTX00951 SIRT1 antibody [GT1189]. Red : Primary antibody Blue : DAPI
SIRT1 antibody [GT1189] - Recombinant antibody, KO/KD, Orthogonal Validated
GTX00951
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSIRT1
Overview
- SupplierGeneTex
- Product NameSIRT1 antibody [GT1189] - Recombinant antibody, KO/KD, Orthogonal Validated
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1189
- ConjugateUnconjugated
- Gene ID23411
- Target nameSIRT1
- Target descriptionsirtuin 1
- Target synonymsNAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; regulatory protein SIR2 homolog 1; SIR2; SIR2alpha; SIR2L1; SIR2-like protein 1; sirtuin type 1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes. Guo Z et al., 2021 Dec, BioengineeredRead more
- T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice. Hsu WH et al., 2020 Jun 5, Aging (Albany NY)Read more