SIRT2 antibody

GeneTex

SIRT2 antibody

9

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.

WB: 1:2,000. ICC/IF: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Polyclonal

Unconjugated

Sirt2

sirtuin 2

5730427M03Rik; mSIR2L2; NAD-dependent deacetylase sirtuin-2; NAD-dependent protein deacetylase sirtuin-2; regulatory protein SIR2 homolog 2; S; SI; silent mating type information regulation 2, (S.cerevisiae, homolog)-like; sirtuin 2; Sir2l; SIR2L2; SIR2-like protein 2

Rabbit

IgG

NAD-dependent protein deacetylase sirtuin-2

SIR2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. SIR2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). The complexes are recruited to their chromosome targets via interactions with DNA-binding proteins, followed by deacetylation of histones H3 and H4. A final step required for telomeric silencing is binding of the complex to the deacetylated histones and recruitment of the telosome to the nuclear periphery. Sir2 protein is an NAD-dependent histone deacetylase, an enzyme that removes acetyl groups from lysine residues of histone proteins and possibly other substrates. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl-ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. The maintenance or silencing of chromatin may be at the center of processes leading to aging of cells and development of cancer.

Human, Mouse

-20°C or -80°C,2°C to 8°C

12352203