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Immunohistochemistry analysis of paraffin-embedded human tonsil using SIRT7 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded human tonsil using SIRT7 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded human tonsil using SIRT7 Polyclonal Antibody at dilution of 1:200.

SIRT7 Polyclonal Antibody

E-AB-70223
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SIRT7 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIRT7 using anti-SIRT7 antibody (PB9358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thyums tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT7 antigen affinity purified polyclonal antibody (Catalog # PB9358) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT7 at approximately 40 kDa. The expected band size for SIRT7 is at 45 kDa.
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