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ProductsBack/SIVA1 / SIVA Antibody (clone OTI1C9)

SIVA1 / SIVA Antibody (clone OTI1C9)

LS-C174829
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSIVA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SIVA1 / SIVA Antibody (clone OTI1C9)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1C9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10572
  • Target name
    SIVA1
  • Target description
    SIVA1 apoptosis inducing factor
  • Target synonyms
    apoptosis regulatory protein Siva; CD27-binding (Siva) protein; CD27BP; SIVA; Siva-1; Siva-2
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIVA/SIVA1 using anti-SIVA/SIVA1 antibody (A05419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIVA/SIVA1 antigen affinity purified polyclonal antibody (Catalog # A05419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIVA/SIVA1 at approximately 19 kDa. The expected band size for SIVA/SIVA1 is at 19 kDa.
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