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ProductsBack/SIX1 Antibody (clone OTI1H4)

SIX1 Antibody (clone OTI1H4)

LS-C173609
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSIX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SIX1 Antibody (clone OTI1H4)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1H4
  • Concentration
    0.77 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6495
  • Target name
    SIX1
  • Target description
    SIX homeobox 1
  • Target synonyms
    BOS3; DFNA23; homeobox protein SIX1; sine oculis homeobox homolog 1; TIP39
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIX1 using anti-SIX1 antibody (PB9889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX1 antigen affinity purified polyclonal antibody (Catalog # PB9889) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIX1 at approximately 37 kDa. The expected band size for SIX1 is at 32 kDa.
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