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Western blot All lanes: SLC13A2 antibody at 1.29microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 65, 70 kDa Observed band size: 65 kDa
Western blot All lanes: SLC13A2 antibody at 1.29microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 65, 70 kDa Observed band size: 65 kDa
Western blot All lanes: SLC13A2 antibody at 1.29microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 65, 70 kDa Observed band size: 65 kDa

SLC13A2 Antibody

CSB-PA618762ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC13A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC13A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9058
  • Target name
    SLC13A2
  • Target description
    solute carrier family 13 member 2
  • Target synonyms
    Na(+)/dicarboxylate cotransporter 1; Na(+)-coupled citrate transporter; NaCT; NADC1; NaDC-1; renal sodium/dicarboxylate cotransporter; SDCT1; solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2; solute carrier family 13 member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13183
  • Protein Name
    Solute carrier family 13 member 2
  • Scientific Description
    Cotransport of sodium ions and dicarboxylates such as succinate and citrate.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NaDC-1/SLC13A2 using anti-NaDC-1/SLC13A2 antibody (A07831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NaDC-1/SLC13A2 antigen affinity purified polyclonal antibody (Catalog # A07831-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NaDC-1/SLC13A2 at approximately 70 kDa. The expected band size for NaDC-1/SLC13A2 is at 64 kDa.
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