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SLC39A7 / ZIP7 Antibody

LS-C817280
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetSLC39A7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC39A7 / ZIP7 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7922
  • Target name
    SLC39A7
  • Target description
    solute carrier family 39 member 7
  • Target synonyms
    AGM9, D6S115E, D6S2244E, H2-KE4, HKE4, KE4, RING5, ZIP7, zinc transporter SLC39A7, HLA class II region expressed gene KE4, Ke4 gene, mouse, human homolog of, histidine-rich membrane protein Ke4, really interesting new gene 5 protein, solute carrier family 39 (zinc transporter), member 7, zinc transporter 7, zrt-, Irt-like protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZIP7/SLC39A7 using anti-ZIP7/SLC39A7 antibody (A07719-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIP7/SLC39A7 antigen affinity purified polyclonal antibody (Catalog # A07719-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIP7/SLC39A7 at approximately 50 kDa. The expected band size for ZIP7/SLC39A7 is at 50 kDa.
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