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ProductsBack/SLIM / FHL1 Antibody (clone OTI2E11)

SLIM / FHL1 Antibody (clone OTI2E11)

LS-C172314
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetFHL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLIM / FHL1 Antibody (clone OTI2E11)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100), IF (1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2E11
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2273
  • Target name
    FHL1
  • Target description
    four and a half LIM domains 1
  • Target synonyms
    FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; four and a half LIM domains protein 1; four-and-a-half Lin11, Isl-1 and Mec-3 domains 1; KYOT; LIM protein SLIMMER; RBMX1A; RBMX1B; skeletal muscle LIM-protein 1; SLIM; SLIM1; SLIM-1; SLIMMER; XMPMA
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FHL using anti-FHL antibody (A01258-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.
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