WB using Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at 1:500. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG.
WB using Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at 1:500. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG.
SMAD1 Rabbit Polyclonal Antibody
TA319239
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetSMAD1
Overview
- SupplierOriGene
- Product NameSMAD1 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID4086
- Target nameSMAD1
- Target descriptionSMAD family member 1
- Target synonymsBSP1; BSP-1; JV41; JV4-1; MAD, mothers against decapentaplegic homolog 1; MADH1; MADR1; Mad-related protein 1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ15797
- Protein NameMothers against decapentaplegic homolog 1
- Scientific DescriptionRabbit polyclonal anti-SMAD1 antibody
- ReactivityCanine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203