Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 (phospho Ser250) antibody [GT1291] (GTX03203) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of 293T cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03203 SMAD2 (phospho Ser250) antibody [GT1291]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03203/GTX03203_78_WB_w_23053123_814.webp "WB analysis of 293T cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03203 SMAD2 (phospho Ser250) antibody [GT1291]. Dilution : 1:1000 Loading : 25microg per lane")
![WB analysis of C2C12 cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03203 SMAD2 (phospho Ser250) antibody [GT1291]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03203/GTX03203_79_WB_w_23053123_818.webp "WB analysis of C2C12 cells were treated by PMA/TPA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03203 SMAD2 (phospho Ser250) antibody [GT1291]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 (phospho Ser250) antibody [GT1291] (GTX03203) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SMAD2 (phospho Ser250) antibody [GT1291] - Orthogonal Validated
GTX03203
Overview
- SupplierGeneTex
- Product NameSMAD2 (phospho Ser250) antibody [GT1291] - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1291
- Concentration0.56 mg/ml
- ConjugateUnconjugated
- Gene ID4087
- Target nameSMAD2
- Target descriptionSMAD family member 2
- Target synonymshMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; MADH2; MADR2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
- ReactivityHuman, Mouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203