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Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SNAI1 antibody

GTX125918
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNAI1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SNAI1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.68 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6615
  • Target name
    SNAI1
  • Target description
    snail family transcriptional repressor 1
  • Target synonyms
    SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1, zinc finger protein SNAI1, protein sna, protein snail homolog 1, snail 1 homolog, snail 1 zinc finger protein, snail family zinc finger 1, snail homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95863
  • Protein Name
    Zinc finger protein SNAI1
  • Scientific Description
    The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu WS, Cheng CC, Lee YH, et al. Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma. Am J Cancer Res. 2023,13(10):4903-4917.
    Read this paper
  • Chen WC, Wu CC, Liu YP, et al. Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling. Cancers (Basel). 2023,15(15). doi: 10.3390/cancers15153825
    Read this paper
  • Hung TH, Huang Y, Yeh CT, et al. High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma. Biomed J. 2024,47(2):100612. doi: 10.1016/j.bj.2023.100612
    Read this paper
  • Wang L, Ye G, Wang Y, et al. Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells. Bioengineered. 2022,13(5):12941-12954. doi: 10.1080/21655979.2022.2079253
    Read this paper
  • Wang LT, Liu KY, Chiou SS, et al. Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance. Cancer Lett. 2021,520:160-171. doi: 10.1016/j.canlet.2021.07.011
    Read this paper
  • Lv F, Du Q, Li L, et al. Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway. Eur J Pharmacol. 2021,900:174069. doi: 10.1016/j.ejphar.2021.174069
    Read this paper
  • Lee KY, Kuo TC, Chou CM, et al. Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling. Cells. 2020,10(1). doi: 10.3390/cells10010028
    Read this paper
  • Jao TM, Fang WH, Ciou SC, et al. PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer. Cancer Lett. 2021,499:290-300. doi: 10.1016/j.canlet.2020.11.017
    Read this paper
  • Kuo CH, Lin MW, Hsu YW, et al. Biomarker discovery in highly invasive lung cancer cell through proteomics approaches. Cell Biochem Funct. 2021,39(3):367-379. doi: 10.1002/cbf.3599
    Read this paper
  • Huang HY, Chou HC, Law CH, et al. Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis. J Cell Mol Med. 2020,24(17):9737-9751. doi: 10.1111/jcmm.15535
    Read this paper

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Figure 1. Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Raji whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa.
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