HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1/2/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1/2/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
SNAI1 Polyclonal Antibody
bs-1371R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSNAI1
Overview
- SupplierBioss
- Product NameSNAI1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6615
- Target nameSNAI1
- Target descriptionsnail family transcriptional repressor 1
- Target synonymsSLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1, zinc finger protein SNAI1, protein sna, protein snail homolog 1, snail 1 homolog, snail 1 zinc finger protein, snail family zinc finger 1, snail homolog 1
- HostRabbit
- IsotypeIgG
- Protein IDO95863
- Protein NameZinc finger protein SNAI1
- Storage Instruction-20°C
- UNSPSC12352203
References
- Li D, Yun Y, Gao R. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019,255(Pt 2):113261. doi: 10.1016/j.envpol.2019.113261Read this paper
- Li Z, Duan H, Li W, et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019,184:227-233. doi: 10.1016/j.exer.2019.04.012Read this paper
- Yang Z, Zhang C, Feng Y, et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma. Exp Mol Pathol. 2019,109:104239. doi: 10.1016/j.yexmp.2019.03.007Read this paper
- Pan S, An L, Meng X, et al. MgCl(2) and ZnCl(2) promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway. Exp Ther Med. 2017,14(5):4663-4670. doi: 10.3892/etm.2017.5144Read this paper
- Chaudhury A, Cheema S, Fachini JM, et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. Nat Commun. 2016,7:13362. doi: 10.1038/ncomms13362Read this paper
- Lin L, Sun Y, Wang D, et al. Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition. Front Pharmacol. 2015,6:320. doi: 10.3389/fphar.2015.00320Read this paper
- Hsu JY, Chang KY, Chen SH, et al. Epidermal growth factor-induced cyclooxygenase-2 enhances head and neck squamous cell carcinoma metastasis through fibronectin up-regulation. Oncotarget. 2015,6(3):1723-39.Read this paper
- Cai J. Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma. Exp Ther Med. 2013,6(6):1489-1493.Read this paper
- Ning Q, Liu C, Hou L, et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. PLoS One. 2013,8(6):e65217. doi: 10.1371/journal.pone.0065217Read this paper
Datasheet
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