HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1/2/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Snail1/2/3 Polyclonal Antibody
bs-1371R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetSNAI2
Overview
- SupplierBioss Antibodies
- Product NameSnail1/2/3 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID6591
- Target nameSNAI2
- Target descriptionsnail family transcriptional repressor 2
- Target synonymsneural crest transcription factor SLUG; protein snail homolog 2; SLUG; slug (chicken homolog), zinc finger protein; SLUGH; SLUGH1; snail family zinc finger 2; snail homolog 2; SNAIL2; WS2D; zinc finger protein SNAI2
- HostRabbit
- IsotypeIgG
- Protein IDP97469
- Protein NameZinc finger protein SNAI2
- ReactivityHuman, Mouse, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Li D et al., 2019 Dec, Environ PollutRead more
- Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Li Z et al., 2019 Jul, Exp Eye ResRead more
- Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1alpha axis in esophageal squamous cell carcinoma. Yang Z et al., 2019 Aug, Exp Mol PatholRead more
- MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/beta-catenin pathway. Pan S et al., 2017 Nov, Exp Ther MedRead more
- CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. Chaudhury A et al., 2016 Nov 21, Nat CommunRead more
- Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition. Lin L et al., 2015, Front PharmacolRead more
- Epidermal growth factor-induced cyclooxygenase-2 enhances head and neck squamous cell carcinoma metastasis through fibronectin up-regulation. Hsu JY et al., 2015 Jan 30, OncotargetRead more
- Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma. Cai J, 2013 Dec, Exp Ther MedRead more
- Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. Ning Q et al., 2013, PLoS OneRead more