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SNAP25 antibody

GTX113839
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetSNAP25
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Overview

  • Supplier
    GeneTex
  • Product Name
    SNAP25 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.27 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6616
  • Target name
    SNAP25
  • Target description
    synaptosome associated protein 25
  • Target synonyms
    bA416N4.2; CMS18; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP; SNAP-25; SUP; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60880
  • Protein Name
    Synaptosomal-associated protein 25
  • Scientific Description
    Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Activation of alpha7 Nicotinic Acetylcholine Receptor by its Selective Agonist Improved Learning and Memory of Amyloid Precursor Protein/Presenilin 1 (APP/PS1) Mice via the Nrf2/HO-1 Pathway. Cao K et al., 2022 Jan 4, Med Sci Monit
    Read more
  • Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons. Liang D et al., 2021 Jul, Protein Cell
    Read more
  • Blood-Brain Barrier Disruption Increases Amyloid-Related Pathology in TgSwDI Mice. Abdallah IM et al., 2021 Jan 27, Int J Mol Sci
    Read more
  • Lowered levels of nicotinic acetylcholine receptors and elevated apoptosis in the hippocampus of brains from patients with type 2 diabetes mellitus and db/db mice. Xu Y et al., 2020 Jul 23, Aging (Albany NY)
    Read more
  • The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of alpha7 nicotinic acetylcholine receptors. Cao K et al., 2020 Jan 30, Aging (Albany NY)
    Read more
  • Activation of alpha7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway. Wang XL et al., 2020 Jan 6, Aging (Albany NY)
    Read more
  • Granisetron Alleviates Alzheimers Disease Pathology in TgSwDI Mice Through Calmodulin-Dependent Protein Kinase II/cAMP-Response Element Binding Protein Pathway. Al Rihani SB et al., 2019, J Alzheimers Dis
    Read more
  • Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Cao K et al., 2019 Apr 22, Alzheimers Res Ther
    Read more
  • Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice. Duong QV et al., 2019 Mar 25, Int J Mol Sci
    Read more
  • Multi-faceted therapeutic strategy for treatment of Alzheimers disease by concurrent administration of etodolac and alpha-tocopherol. Elfakhri KH et al., 2019 May, Neurobiol Dis
    Read more

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Figure 1. Western blot analysis of SNAP25 using anti-SNAP25 antibody (A01625-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (Catalog # A01625-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 24 kDa. The expected band size for SNAP25 is at 24 kDa.
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