SNAP25 Polyclonal Antibody

Reddot Biotech

SNAP25 Polyclonal Antibody

5

Western Blot

Research Use Only

Polyclonal

2 mg/ml

Unconjugated

Snap25

synaptosomal-associated protein 25

Bd; Bdr; blind drunk; GENA70; SNA; SNAP-25; sp; SUP; super protein; synaptosomal-associated 25 kDa protein; synaptosomal-associated protein 25; synaptosomal-associated protein, 25 kDa

Rabbit

IgG

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay,it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition,it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide,probably by initiating the formation of a fusion competent SNARE complex.

Mouse

-20°C

12352203