SOD1 ELISA Pair
GTX500010
ApplicationsELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOD1
Overview
- SupplierGeneTex
- Product NameSOD1 ELISA Pair
- Delivery Days Customer9
- Application Supplier NoteWe recommend using the capture antibody at a concentration of 5microg/mL and the detection antibody at a concentration of 1.5microg/mL. Optimal dilutions should be determined experimentally by the researcher.
- ApplicationsELISA
- CertificationResearch Use Only
- Gene ID6647
- Target nameSOD1
- Target descriptionsuperoxide dismutase 1
- Target synonymsALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer, superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SOD, soluble, epididymis secretory protein Li 44, indophenoloxidase A, superoxide dismutase 1, soluble, superoxide dismutase, cystolic
- Scientific DescriptionThe protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203


![ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT.
Permeabilization : 0.2% Triton X-100 for 5 min
Dilution : 1μg/ml (12 hours at 4oC)](https://www.genetex.com/upload/website/prouct_img/normal/GTX57211/GTX57211_GTX57211_ICC_IF_1-1_w_23061123_271.webp)
![IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”.
Antibody amount : MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg
IP lysis buffer: 1% Triton X100/TBS buffer.](https://www.genetex.com/upload/website/prouct_img/normal/GTX57212/GTX57212_IP_1-1_w_23061123_188.webp)

![SOD1 antibody [HL1652] detects SOD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637250/GTX637250_T-44753_20220805_IHC-P_R_22080923_144.webp)
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1653] (GTX637251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637251/GTX637251_T-44753_20220729_WB_M_R_22080119_341.webp)
![SOD1 antibody [HL2033] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SOD1 stained by SOD1 antibody [HL2033] (GTX637934) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637934/GTX637934_T-44879_20230106_IHC-P_M_23010400_601.webp)
![ICC/IF analysis of PANC-1 (left) and SKBR-3 (right) cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX83012/GTX83012_20170912_ICCIF_1_w_23061322_886.webp)