IHC-P analysis of human colon carcinoma tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human kidney tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22872/GTX22872_1190_IHC-P_w_23060620_889.webp "IHC-P analysis of human kidney tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![IHC-P analysis of human tonsil tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX22872/GTX22872_1191_IHC-P_w_23060620_592.webp "IHC-P analysis of human tonsil tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50")
![WB analysis of 25 ug of rat kidney (lane 1) and mouse kidney (lane 2) using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22872/GTX22872_1619_WB_w_23060620_646.webp "WB analysis of 25 ug of rat kidney (lane 1) and mouse kidney (lane 2) using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Dilution : 1:1000")
![ICC/IF analysis of HeLa cells using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22872/GTX22872_532_ICC-IF_w_23060620_863.webp "ICC/IF analysis of HeLa cells using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 for at least 1 hour at room temperature")
IHC-P analysis of human colon carcinoma tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]
GTX22872
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityCanine, Human, Mouse, Porcine, Primate, Rat, Sheep, Yeast
TargetATP1A1
Overview
- SupplierGeneTex
- Product NameSodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.
- Application Supplier NoteWB: 1:200-1:2000. ICC/IF: 1/50 - 1/200. IHC-P: 1/100 - 1/2000. IHC-Fr: 1:100. FACS: 1/20 - 1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDM8-P1-A3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID476
- Target nameATP1A1
- Target descriptionATPase Na+/K+ transporting subunit alpha 1
- Target synonymsATPase, Na+/K+ transporting, alpha 1 polypeptide; CMT2DD; HOMGSMR2; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na+/K+ ATPase 1; sodium pump subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide
- HostMouse
- IsotypeIgG1
- Protein IDP05023
- Protein NameSodium/potassium-transporting ATPase subunit alpha-1
- Scientific DescriptionThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
- ReactivityCanine, Human, Mouse, Porcine, Primate, Rat, Sheep, Yeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Reinhard JR et al., 2016 May 19, Nat CommunRead more