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ProductsBack/SOX17 Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SOX17 antibody (TA381894) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 2s.
Western blot analysis of extracts of various cell lines, using SOX17 antibody (TA381894) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 2s.
Western blot analysis of extracts of various cell lines, using SOX17 antibody (TA381894) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 2s.

SOX17 Rabbit Polyclonal Antibody

TA381894
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetSOX17
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SOX17 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID64321
  • Target name
    SOX17
  • Target description
    SRY-box transcription factor 17
  • Target synonyms
    PPH7, VUR3, transcription factor SOX-17, SRY (sex determining region Y)-box 17, SRY-box 17, SRY-related HMG-box transcription factor SOX17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6I2
  • Protein Name
    Transcription factor SOX-17
  • Scientific Description
    SOX17 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SOX17 using anti-SOX17 antibody (A01665-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX17 antigen affinity purified polyclonal antibody (Catalog # A01665-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX17 at approximately 45 kDa. The expected band size for SOX17 is at 45 kDa.
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