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ProductsBack/SOX17 Rabbit Polyclonal Antibody
Western blot analysis of SOX17 in Jurkat lysates using Sox17 antibody.
Western blot analysis of SOX17 in Jurkat lysates using Sox17 antibody.
Western blot analysis of SOX17 in Jurkat lysates using Sox17 antibody.

SOX17 Rabbit Polyclonal Antibody

TA385303
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSOX17
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SOX17 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID64321
  • Target name
    SOX17
  • Target description
    SRY-box transcription factor 17
  • Target synonyms
    PPH7, VUR3, transcription factor SOX-17, SRY (sex determining region Y)-box 17, SRY-box 17, SRY-related HMG-box transcription factor SOX17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6I2
  • Protein Name
    Transcription factor SOX-17
  • Scientific Description
    SOX17 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SOX17 using anti-SOX17 antibody (A01665-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX17 antigen affinity purified polyclonal antibody (Catalog # A01665-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX17 at approximately 45 kDa. The expected band size for SOX17 is at 45 kDa.
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