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WB analysis of 293T cells using GTX45695 SPATA5 antibody at 0.2-1microg/ml.
WB analysis of 293T cells using GTX45695 SPATA5 antibody at 0.2-1microg/ml.
WB analysis of 293T cells using GTX45695 SPATA5 antibody at 0.2-1microg/ml.

SPATA5 antibody, Internal

GTX45695
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSPATA5
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Overview

  • Supplier
    GeneTex
  • Product Name
    SPATA5 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID166378
  • Target name
    SPATA5
  • Target description
    spermatogenesis associated 5
  • Target synonyms
    AFG2; ATPase family gene 2 homolog; ATPase family protein 2 homolog; EHLMRS; SPAF; spermatogenesis associated factor SPAF; spermatogenesis-associated factor protein; spermatogenesis-associated protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB90
  • Protein Name
    Spermatogenesis-associated protein 5
  • Scientific Description
    This gene encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in this gene have been associated with epilepsy, hearing loss, and cognitive disability syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPATA5 using anti-SPATA5 antibody (A06364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA5 antigen affinity purified polyclonal antibody (Catalog # A06364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA5 at approximately 98 kDa. The expected band size for SPATA5 is at 98 kDa.
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