SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230519_WB_M_23053001_287.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230519_WB_TPM_watermark_23053001_386.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638281/GTX638281_45040_20230519_WB_R_23053001_153.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230630_ICC_IF_23071123_776.webp "SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SPG7 antibody [HL2236]
GTX638281
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSPG7
Overview
- SupplierGeneTex
- Product NameSPG7 antibody [HL2236]
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:20-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2236
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6687
- Target nameSPG7
- Target descriptionSPG7 matrix AAA peptidase subunit, paraplegin
- Target synonymsCAR, CMAR, PGN, SPG5C, mitochondrial inner membrane m-AAA protease component paraplegin, SPG7, paraplegin matrix AAA peptidase subunit, cell matrix adhesion regulator, spastic paraplegia 7 (pure and complicated autosomal recessive), spastic paraplegia 7 protein
- HostRabbit
- IsotypeIgG
- Protein IDQ9UQ90
- Protein NameMitochondrial inner membrane m-AAA protease component paraplegin
- Scientific DescriptionThis gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
Related products
Product group Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPG7
- SizePrice
Product group Antibodies
Anti-SPG7 Antibody144-10249
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSPG7
- SizePrice
Product group Antibodies
Paraplegin / SPG7 AntibodyLS-C496959
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSPG7
- SizePrice
Product group Antibodies
SPG7 antibodyGTX64687
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetSPG7
- SizePrice