SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![SQSTM1 / P62 (phospho Ser403) antibody detects SQSTM1 / P62 (phospho Ser403) protein at autophagosome? by immunofluorescent analysis. Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SQSTM1 / P62 (phospho Ser403) stained by SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX128171/GTX128171_43194_20181205_ICC_IF_Thapsigargin_w_23060523_611.webp "SQSTM1 / P62 (phospho Ser403) antibody detects SQSTM1 / P62 (phospho Ser403) protein at autophagosome? by immunofluorescent analysis. Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SQSTM1 / P62 (phospho Ser403) stained by SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.")
SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
SQSTM1 / P62 (phospho Ser403) antibody
GTX128171
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSQSTM1
Overview
- SupplierGeneTex
- Product NameSQSTM1 / P62 (phospho Ser403) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.08 mg/ml
- ConjugateUnconjugated
- Gene ID8878
- Target nameSQSTM1
- Target descriptionsequestosome 1
- Target synonymsA170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1, EBI3-associated protein of 60 kDa, EBI3-associated protein p60, autophagy receptor p62, oxidative stress induced like, phosphotyrosine independent ligand for the Lck SH2 domain p62, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62
- HostRabbit
- IsotypeIgG
- Protein IDQ13501
- Protein NameSequestosome-1
- Scientific DescriptionThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ikeda R, Noshiro D, Morishita H, et al. Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response. EMBO J. 2023,42(14):e113349. doi: 10.15252/embj.2022113349Read this paper
- Porras G, Ruiz S, Maestro I, et al. Functional Characterization of a Familial ALS-Associated Missense TBK1 (p-Arg573Gly) Mutation in Patient-Derived Lymphoblasts. Int J Mol Sci. 2023,24(3). doi: 10.3390/ijms24032847Read this paper
- Liu Y, Deng H, Liang L, et al. Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Genes Dis. 2021,8(2):232-240. doi: 10.1016/j.gendis.2020.07.009Read this paper
- Kageyama S, Gudmundsson SR, Sou YS, et al. p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response. Nat Commun. 2021,12(1):16. doi: 10.1038/s41467-020-20185-1Read this paper
- El-Derany MO, El-Demerdash E. Pyrvinium pamoate attenuates non-alcoholic steatohepatitis: Insight on hedgehog/Gli and Wnt/β-catenin signaling crosstalk. Biochem Pharmacol. 2020,177:113942. doi: 10.1016/j.bcp.2020.113942Read this paper
- Bondad SEC, Kurasaki M. Analysis of Cadmium, Epigallocatechin Gallate, and Vitamin C Co-exposure on PC12 Cellular Mechanisms. Biol Trace Elem Res. 2020,198(2):627-635. doi: 10.1007/s12011-020-02097-9Read this paper
- Lee S, Jeon YM, Cha SJ, et al. PTK2/FAK regulates UPS impairment via SQSTM1/p62 phosphorylation in TARDBP/TDP-43 proteinopathies. Autophagy. 2020,16(8):1396-1412. doi: 10.1080/15548627.2019.1686729Read this paper
- Qiu Y, Yao J, Jia L, et al. Shifting the balance of autophagy and proteasome activation reduces proteotoxic cell death: a novel therapeutic approach for restoring photoreceptor homeostasis. Cell Death Dis. 2019,10(8):547. doi: 10.1038/s41419-019-1780-1Read this paper
- Ding X, Jiang X, Tian R, et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 2019,15(10):1774-1786. doi: 10.1080/15548627.2019.1596478Read this paper
- Catanese A, Olde Heuvel F, Mulaw M, et al. Retinoic acid worsens ATG10-dependent autophagy impairment in TBK1-mutant hiPSC-derived motoneurons through SQSTM1/p62 accumulation. Autophagy. 2019,15(10):1719-1737. doi: 10.1080/15548627.2019.1589257Read this paper
Datasheet
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