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ProductsBack/SQSTM1 / P62 (phospho Ser403) antibody

SQSTM1 / P62 (phospho Ser403) antibody

GTX128171
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSQSTM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SQSTM1 / P62 (phospho Ser403) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.08 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8878
  • Target name
    SQSTM1
  • Target description
    sequestosome 1
  • Target synonyms
    A170; autophagy receptor p62; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; OSIL; oxidative stress induced like; p60; p62; p62B; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; sequestosome-1; ubiquitin-binding protein p62; ZIP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13501
  • Protein Name
    Sequestosome-1
  • Scientific Description
    This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response.
    Read more
  • Functional Characterization of a Familial ALS-Associated Missense TBK1 (p-Arg573Gly) Mutation in Patient-Derived Lymphoblasts.
    Read more
  • Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Liu Y et al., 2021 Mar, Genes Dis
    Read more
  • p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response. Kageyama S et al., 2021 Jan 4, Nat Commun
    Read more
  • Pyrvinium pamoate attenuates non-alcoholic steatohepatitis: Insight on hedgehog/Gli and Wnt/beta-catenin signaling crosstalk. El-Derany MO et al., 2020 Jul, Biochem Pharmacol
    Read more
  • Analysis of Cadmium, Epigallocatechin Gallate, and Vitamin C Co-exposure on PC12 Cellular Mechanisms. Bondad SEC et al., 2020 Dec, Biol Trace Elem Res
    Read more
  • PTK2/FAK regulates UPS impairment via SQSTM1/p62 phosphorylation in TARDBP/TDP-43 proteinopathies. Lee S et al., 2020 Aug, Autophagy
    Read more
  • Shifting the balance of autophagy and proteasome activation reduces proteotoxic cell death: a novel therapeutic approach for restoring photoreceptor homeostasis. Qiu Y et al., 2019 Jul 18, Cell Death Dis
    Read more
  • RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Ding X et al., 2019 Oct, Autophagy
    Read more
  • Retinoic acid worsens ATG10-dependent autophagy impairment in TBK1-mutant hiPSC-derived motoneurons through SQSTM1/p62 accumulation. Catanese A et al., 2019 Oct, Autophagy
    Read more

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Figure 1. Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 antigen affinity purified polyclonal antibody (Catalog # PB9444) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 62 kDa. The expected band size for SQSTM1 is at 48 kDa.
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