Untreated (-) and treated (+) NIH-3T3 whole cell extracts (15 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr416) antibody [GT1314] (GTX03226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of NIH-3T3 cells were treated by pervanadate (1 mM) at 37oC for 30 minutes after serum-starvation overnight using GTX03226 Src (phospho Tyr419) antibody [GT1314]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03226/GTX03226_89_WB_w_23053123_444.webp "WB analysis of NIH-3T3 cells were treated by pervanadate (1 mM) at 37oC for 30 minutes after serum-starvation overnight using GTX03226 Src (phospho Tyr419) antibody [GT1314]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) NIH-3T3 whole cell extracts (15 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr416) antibody [GT1314] (GTX03226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Src (phospho Tyr416) antibody [GT1314]
GTX03226
Overview
- SupplierGeneTex
- Product NameSrc (phospho Tyr416) antibody [GT1314] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityBased on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1314
- Concentration0.19 mg/ml
- ConjugateUnconjugated
- Gene ID6714
- Target nameSRC
- Target descriptionSRC proto-oncogene, non-receptor tyrosine kinase
- Target synonymsASV; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; proto-oncogene tyrosine-protein kinase Src; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
- HostRabbit
- IsotypeIgG
- Protein IDP12931
- Protein NameProto-oncogene tyrosine-protein kinase Src
- Scientific DescriptionThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203