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ProductsBack/STAM1 (STAM) Rabbit Polyclonal Antibody
WB Suggested Anti-STAM Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellSTAM is supported by BioGPS gene expression data to be expressed in HeLa
WB Suggested Anti-STAM Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellSTAM is supported by BioGPS gene expression data to be expressed in HeLa
WB Suggested Anti-STAM Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellSTAM is supported by BioGPS gene expression data to be expressed in HeLa

STAM1 (STAM) Rabbit Polyclonal Antibody

TA358344
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetSTAM
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    STAM1 (STAM) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8027
  • Target name
    STAM
  • Target description
    signal transducing adaptor molecule
  • Target synonyms
    HSE1 homolog; signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM1; STAM-1
  • Host
    Rabbit
  • Protein IDQ92783
  • Protein Name
    Signal transducing adapter molecule 1
  • Scientific Description
    STAM Antibody - N-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAM1 using anti-STAM1 antibody (A00864-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAM1 antigen affinity purified polyclonal antibody (Catalog # A00864-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAM1 at approximately 59KD. The expected band size for STAM1 is at 59KD.
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