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Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

STAT3 (phospho Tyr705) antibody

GTX118000
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Zebra Fish
TargetSTAT3
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Overview

  • Supplier
    GeneTex
  • Product Name
    STAT3 (phospho Tyr705) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6774
  • Target name
    STAT3
  • Target description
    signal transducer and activator of transcription 3
  • Target synonyms
    ADMIO, ADMIO1, APRF, HIES, signal transducer and activator of transcription 3, DNA-binding protein APRF, acute-phase response factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40763
  • Protein Name
    Signal transducer and activator of transcription 3
  • Scientific Description
    The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wang BY, Shen HT, Lee YL, et al. Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression. Am J Cancer Res. 2023,13(10):4721-4733.
    Read this paper
  • Zhao M, Zheng Z, Zhang P, et al. IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis. iScience. 2023,26(9):107544. doi: 10.1016/j.isci.2023.107544
    Read this paper
  • Di Giorgio C, Bellini R, Lupia A, et al. Discovery of BAR502, as potent steroidal antagonist of leukemia inhibitory factor receptor for the treatment of pancreatic adenocarcinoma. Front Oncol. 2023,13:1140730. doi: 10.3389/fonc.2023.1140730
    Read this paper
  • Di Giorgio C, Lupia A, Marchianò S, et al. Repositioning Mifepristone as a Leukaemia Inhibitory Factor Receptor Antagonist for the Treatment of Pancreatic Adenocarcinoma. Cells. 2022,11(21). doi: 10.3390/cells11213482
    Read this paper
  • Di Giorgio C, Marchianò S, Marino E, et al. Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis. Front Oncol. 2022,12:939969. doi: 10.3389/fonc.2022.939969
    Read this paper
  • Lin ZH, Hu J, Shi H, et al. Water extract of medicinal ink (WEMI) attenuates lipopolysaccharide-induced NO production of Raw264.7 cells via downregulating JAK2/STAT3-mediated iNOS expression. J Ethnopharmacol. 2022,282:114636. doi: 10.1016/j.jep.2021.114636
    Read this paper
  • Zhang L, Sui C, Zhang Y, et al. Knockdown of hsa_circ_0134111 alleviates the symptom of osteoarthritis via sponging microRNA-224-5p. Cell Cycle. 2021,20(11):1052-1066. doi: 10.1080/15384101.2021.1919838
    Read this paper
  • Wu SY, Yang WY, Cheng CC, et al. Low molecular weight fucoidan inhibits hepatocarcinogenesis and nonalcoholic fatty liver disease in zebrafish via ASGR/STAT3/HNF4A signaling. Clin Transl Med. 2020,10(8):e252. doi: 10.1002/ctm2.252
    Read this paper
  • Castañeda-Zárraga A, Rodríguez-Cid JR, Flores-Mariñelarena RR, et al. Human skin biomarkers relationship to response to treatment with tyrosine kinase inhibitors in advanced EGFR-mutated lung adenocarcinoma. Thorac Cancer. 2020,11(11):3243-3251. doi: 10.1111/1759-7714.13657
    Read this paper
  • Wang YY, Xiao LY, Chen YK, et al. Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo. ACS Omega. 2020,5(12):7018-7024. doi: 10.1021/acsomega.0c00640
    Read this paper

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