Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-S727 antibody (TA382094) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-S727 antibody (TA382094) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 1s.
STAT3 Rabbit Polyclonal Antibody
TA382094
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT3
Overview
- SupplierOriGene
- Product NameSTAT3 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID6774
- Target nameSTAT3
- Target descriptionsignal transducer and activator of transcription 3
- Target synonymsacute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; HIES; signal transducer and activator of transcription 3
- HostRabbit
- IsotypeIgG
- Protein IDP40763
- Protein NameSignal transducer and activator of transcription 3
- Scientific DescriptionPhospho-STAT3-S727 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203