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ProductsBack/STAT6 Antibody (clone 7D3)

STAT6 Antibody (clone 7D3)

LS-C169219
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetSTAT6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    STAT6 Antibody (clone 7D3)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), IF (1:200 - 1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7D3
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6778
  • Target name
    STAT6
  • Target description
    signal transducer and activator of transcription 6
  • Target synonyms
    D12S1644; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT6 using anti-STAT6 antibody (A00523-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified polyclonal antibody (Catalog # A00523-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 110KD. The expected band size for STAT6 is at 110KD.
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