Streptavidin (HRP)
GTX85912
Product group Molecular Biology
Overview
- SupplierGeneTex
- Product NameStreptavidin (HRP)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- Concentration1 mg/ml
- ConjugateHRP
- Scientific DescriptionStreptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.
- Storage Instruction2°C to 8°C
- UNSPSC41116133
References
- Parasar B, Chang PV. BSH-TRAP: Bile salt hydrolase tagging and retrieval with activity-based probes. Methods Enzymol. 2022,664:85-102. doi: 10.1016/bs.mie.2021.12.002Read this paper
- Nanashima N, Horie K, Maeda H, et al. Blackcurrant Anthocyanins Increase the Levels of Collagen, Elastin, and Hyaluronic Acid in Human Skin Fibroblasts and Ovariectomized Rats. Nutrients. 2018,10(4). doi: 10.3390/nu10040495Read this paper
- Kung YA, Hung CT, Chien KY, et al. Control of the negative IRES trans-acting factor KHSRP by ubiquitination. Nucleic Acids Res. 2017,45(1):271-287. doi: 10.1093/nar/gkw1042Read this paper