ICC/IF analysis of COS7 cells transiently transfected with Survivin plasmid using GTX84824 Survivin antibody [2D9].
![IHC-P analysis of human kidney tissue using GTX84824 Survivin antibody [2D9]. Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120oC for 3min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84824/GTX84824_3145_IHC-P_w_23061420_181.webp "IHC-P analysis of human kidney tissue using GTX84824 Survivin antibody [2D9]. Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120oC for 3min. Dilution : 1:50")
![IHC-P analysis of human liver tissue using GTX84824 Survivin antibody [2D9]. Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120oC for 3min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84824/GTX84824_3146_IHC-P_w_23061420_687.webp "IHC-P analysis of human liver tissue using GTX84824 Survivin antibody [2D9]. Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120oC for 3min. Dilution : 1:50")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Uterus ; Lane 3-Breast ; Lane 4-Brain ; Lane 5-Liver ; Lane 6-Ovary ; Lane 7-Thyroid gland ; Lane 8-colon) using GTX84824 Survivin antibody [2D9]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84824/GTX84824_4270_WB_w_23061420_331.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Uterus ; Lane 3-Breast ; Lane 4-Brain ; Lane 5-Liver ; Lane 6-Ovary ; Lane 7-Thyroid gland ; Lane 8-colon) using GTX84824 Survivin antibody [2D9]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with Survivin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84824 Survivin antibody [2D9]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84824/GTX84824_4648_WB_w_23061420_917.webp "WB analysis of HEK293T cells transfected with Survivin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84824 Survivin antibody [2D9]. Loading : 5 ug per lane")
![FACS analysis of HEK293T cells transfected with either Survivin plasmid(Red) or empty vector control plasmid(Blue) using GTX84824 Survivin antibody [2D9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84824/GTX84824_564_FACS_w_23061420_118.webp "FACS analysis of HEK293T cells transfected with either Survivin plasmid(Red) or empty vector control plasmid(Blue) using GTX84824 Survivin antibody [2D9].")
ICC/IF analysis of COS7 cells transiently transfected with Survivin plasmid using GTX84824 Survivin antibody [2D9].
Survivin antibody [2D9]
GTX84824
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBIRC5
Overview
- SupplierGeneTex
- Product NameSurvivin antibody [2D9] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2D9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID332
- Target nameBIRC5
- Target descriptionbaculoviral IAP repeat containing 5
- Target synonymsAPI4; apoptosis inhibitor 4; apoptosis inhibitor survivin; baculoviral IAP repeat-containing protein 5; EPR-1; survivin variant 3 alpha
- HostMouse
- IsotypeIgG1
- Protein IDO15392
- Protein NameBaculoviral IAP repeat-containing protein 5
- Scientific DescriptionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203