SYBR Green Fluorescent DNA Stain

Biorbyt

SYBR Green Fluorescent DNA Stain

16

Spectroscopic Propertie: lambdaexc 495 nm, lambdaem 520 nm (bound to DNA).

Research Use Only

100 uM

SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. SYBR® Green Fluorescent DNA Stain is supplied as 100 microM con-centration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 microM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument. final SYBR® Green concentration20 microl PCR assay 50 microl PCR assay 0.5 microM 0.1 microl 0.25 microl 1.0 microM 0.2 microl 0.50 microl SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.

-20°C

41105300