WB of rat brain lysate showing detection of ~65 kDa Synaptotagmin 7 protein using Anti-Synaptotagmin 7. Lane 1: MW ladder. Lane 2: Anti-Synaptotagmin 7 (1:100). Lane 3: Anti-Synaptotagmin 7 (1:250). Lane 4: Anti-Synaptotagmin 7 (1:500). Lane 5: Anti-Synaptotagmin 7 (1:1000). Load: 20 ug RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-Synaptotagmin 7 monoclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 65 kDa. Other band (s) (faint): ~25kDa, ~37kDa, ~100kDa, ~150kDa.