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SYTL2 / SLP2 Antibody (aa840-868)

LS-C325535
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSYTL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SYTL2 / SLP2 Antibody (aa840-868)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:1000), Flo (1:10 - 1:50), IHC (1:50 - 1:100), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54843
  • Target name
    SYTL2
  • Target description
    synaptotagmin like 2
  • Target synonyms
    breast cancer-associated antigen SGA-72M; CHR11SYT; chromosome 11 synaptotagmin; EXO4; exophilin-4; PPP1R151; protein phosphatase 1, regulatory subunit 151; SGA72M; SLP2; SLP2A; synaptotagmin-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLP2/SYTL2 using anti-SLP2/SYTL2 antibody (A08255-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLP2/SYTL2 antigen affinity purified polyclonal antibody (Catalog # A08255-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLP2/SYTL2 at approximately 120-130 kDa. The expected band size for SLP2/SYTL2 is at 105 kDa.
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