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ProductsBack/T Box Brain Protein 1 Antibody (YA3217)

T Box Brain Protein 1 Antibody (YA3217)

HY-P83472
MedChem Express
TargetTBR1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    T Box Brain Protein 1 Antibody (YA3217)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10716
  • Target name
    TBR1
  • Target description
    T-box brain transcription factor 1
  • Target synonyms
    IDDAS; T-box brain protein 1; T-box, brain 1; TBR-1; T-brain-1; TES-56
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    T Box Brain Protein 1 Antibody (YA3217) is a non-conjugated IgG antibody, targeting T Box Brain Protein 1, with a predicted molecular weight of 74 kDa (observed band size: 74 kDa). T Box Brain Protein 1 Antibody (YA3217) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TBR1 using anti-TBR1 antibody (A07503-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBR1 antigen affinity purified polyclonal antibody (Catalog # A07503-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBR1 at approximately 74 kDa. The expected band size for TBR1 is at 74 kDa.
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