T4 DNA Ligase

Applied Biological Materials

T4 DNA Ligase

9

Research Use Only

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3- hydroxyl and 5- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

-20°C

12352204