TAG-72 Antibody Cocktail

Biorbyt

TAG-72 Antibody Cocktail

16

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.1. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT IHC-P

Research Use Only

Monoclonal

B72.3 + CA72/733

Unconjugated

tag-72

mRNA cap guanine-N7 methyltransferase

CELE_C25A1.3; mRNA cap guanine-N7 methyltransferase

Mouse

IgG1

This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 antibody with a GCDFP-15 antibody is valuable in the diagnosis of apocrine carcinoma.

Human

-20°C,2°C to 8°C

12352203