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Tag antibodies

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Immunoprecipitation (IP) experiments were performed with negative control lysate (Cat# LY40001) generated by transfecting HEK293T cells with pCMV-entry empty vector (Cat# PS100001), and with overexpression lysate (Cat# LY400339) generated by transfecting TrueORF Gold cDNA clone myc-DDK tagged PON3 (Cat# RC210019) to HEK293T cells. Please see datasheet for details.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
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HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA150052) (1:2000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA150052) (1:2000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with pCMV6-AC-GFPvector (control plasmid, Lane 1) or Myc-DDK-tagged ORF clone of human MC2R gene (Cat# RC210483, Lane 2) plasmids for 48 hrs. Eqivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myc tag-HRP. Conditio: 1:1000 dilution.
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY eGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-eGFP polyclonal antibody (TA150074) (1:3000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY turboGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-turboGFP polyclonal antibody (TA150075) (1:3000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY mKate (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-mKate polyclonal antibody (TA150076) (1:3000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY mPlum (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-mPlum polyclonal antibody (TA150077) (1:3000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
Purified N-terminal DDK fused protein (Left lane) and lysate from HEK293 cells transfected with RC209482 containing C-termnal DDK tag (right lane) were seperated with SDS-PAGE gel and immunobloted with chicken anti-DDK tag polyclonal antibody (TA150079, 1:4000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells transfected with N-terminal Myc tag TDO2 cDNA clone vector (Left lane) or with RC209482 containing C-termnal Myc tag (right lane) were seperated with SDS-PAGE gel and immunobloted withchicken anti-Myc tag polyclonal antibody (TA150082, 1:1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
HEK293T cells transfected with NUP85 cDNA clone vector containing C-termnal HA tag were seperated with SDS-PAGE gel and immunobloted with chicken anti-HA tag polyclonal antibody (TA150085, 1:1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
Anti-V5 immunoblot analysis of six different HEK293T over-expression lysates containing different V5 tagged recombinant proteins at 1:2000 dilution.
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice