Talin antibody [8D4]
![Research Use Only](static/images/certificates/ruo.jpg)
GTX11188
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
Product group Antibodies
ReactivityChicken, Gerbil, Human, Mouse, Rat, Xenopus
TargetTLN1
Overview
- SupplierGeneTex
- Product NameTalin antibody [8D4]
- Delivery Days Customer9
- Antibody SpecificityReacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular
- Application Supplier NoteWB: 1:100. ICC/IF: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8D4
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID395194
- Target nameTLN1
- Target descriptiontalin 1
- Target synonymstalin-1
- HostMouse
- IsotypeIgG1
- Protein IDP54939
- Protein NameTalin-1
- Scientific DescriptionTalin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.
- ReactivityChicken, Gerbil, Human, Mouse, Rat, Xenopus
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions. Xie Y et al., 2019 Jul 15, Anal BiochemRead more
- Inhibition of cell migration by focal adhesion kinase: Time-dependent difference in integrin-induced signaling between endothelial and hepatoblastoma cells. Yu H et al., 2018 May, Int J Mol MedRead more
- A Perturbation Analysis to Understand the Mechanism How Migrating Cells Sense and Respond to a Topography in the Extracellular Environment. Miyoshi H et al., 2016, Anal SciRead more
- alphaV-integrins are required for mechanotransduction in MDCK epithelial cells. Teraevaeinen TP et al., 2013, PLoS OneRead more