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Talin antibody [8D4]

Research Use Only
GTX11188
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
Product group Antibodies
ReactivityChicken, Gerbil, Human, Mouse, Rat, Xenopus
TargetTLN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Talin antibody [8D4]
  • Delivery Days Customer
    9
  • Antibody Specificity
    Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular
  • Application Supplier Note
    WB: 1:100. ICC/IF: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8D4
  • Conjugate
    Unconjugated
  • Formulation
    Liquid
  • Gene ID395194
  • Target name
    TLN1
  • Target description
    talin 1
  • Target synonyms
    talin-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP54939
  • Protein Name
    Talin-1
  • Scientific Description
    Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.
  • Reactivity
    Chicken, Gerbil, Human, Mouse, Rat, Xenopus
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions. Xie Y et al., 2019 Jul 15, Anal Biochem
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  • Inhibition of cell migration by focal adhesion kinase: Time-dependent difference in integrin-induced signaling between endothelial and hepatoblastoma cells. Yu H et al., 2018 May, Int J Mol Med
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  • A Perturbation Analysis to Understand the Mechanism How Migrating Cells Sense and Respond to a Topography in the Extracellular Environment. Miyoshi H et al., 2016, Anal Sci
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  • alphaV-integrins are required for mechanotransduction in MDCK epithelial cells. Teraevaeinen TP et al., 2013, PLoS One
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