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TAMRA phosphoramidite, 5-isomer

TAMRA phosphoramidite, 5-isomer

A270325
Antibodies.com
Estimated Purity95%
Product group Chemicals
Molecular Weight727.83 kDa
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    TAMRA phosphoramidite, 5-isomer
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Estimated Purity
    95%
  • Molecular Formula
    C40H50N5O6P
  • Molecular Weight
    727.83 kDa
  • Scientific Description
    This phosphoramidite is used for synthesis of oligonucleotides 5-labeled with TAMRA. TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5-terminal TAMRA and FAM in the middle of the sequence or at the 3-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively). TAMRA 5-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently. The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection. Coupling takes only 7.5 minutes. Deprotection should be performed with tret-buthylamine : methanol : water at a ratio of 1 : 1 : 3 (v/v/v) (TAMRA cocktail) for 6 hours at 60 °C, then cooled down to room temperature. Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
  • UNSPSC
    41116134