TEV Protease (recombinant)

InVivo Biotech

TEV Protease (recombinant)

5

Research Use Only

Recombinant form of the TEV-Protease from Tobacco Etch Virus produced in Escherichia coli. TEV-Protease is a highly specific cysteine protease with specificity for the sequence ENLYFQS (cleavage position indicated by a down arrow); however, the amino acid in the P1 position can also be G, A, M, C, or H [1]. The expression construct contains residues 2038 to 2279 of the TEV Genome polyprotein (UniProt entry P04517), a natural cleavage product, called Nuclear inclusion protein A (Nia), with a length of 272 amino acids. The protein includes a C-terminal V5-tag (GKPIPNPLLGLDST) and hexa-histidine-tag. The recombinant protein is produced with an E. coli expression system and purified using affinity chromatography and size exclusion chromatography.

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