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Western Blot analysis of various samples using TGF beta Receptor I Polyclonal Antibody at dilution of 1:750.
Western Blot analysis of various samples using TGF beta Receptor I Polyclonal Antibody at dilution of 1:750.
Western Blot analysis of various samples using TGF beta Receptor I Polyclonal Antibody at dilution of 1:750.

TGF beta Receptor I Polyclonal Antibody

E-AB-70130
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetTGFBR1
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Overview

  • Supplier
    Elabscience
  • Product Name
    TGF beta Receptor I Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.9 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7046
  • Target name
    TGFBR1
  • Target description
    transforming growth factor beta receptor 1
  • Target synonyms
    AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I, TGF-beta receptor type-1, activin A receptor type II-like kinase, 53kDa, activin A receptor type II-like protein kinase of 53kD, activin receptor-like kinase 5, mutant transforming growth factor beta receptor I, serine/threonine-protein kinase receptor R4, transforming growth factor beta receptor I, transforming growth factor-beta receptor type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36897
  • Protein Name
    TGF-beta receptor type-1
  • Scientific Description
    The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of TGF Beta Receptor I/TGFBR1 using anti-TGF Beta Receptor I/TGFBR1 antibody (A00832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor I/TGFBR1 antigen affinity purified polyclonal antibody (Catalog # A00832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor I/TGFBR1 at approximately 56 kDa. The expected band size for TGF Beta Receptor I/TGFBR1 is at 56 kDa.
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