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Western Blot analysis of NIH-3T3 cells using Hic-5 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using Hic-5 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using Hic-5 Polyclonal Antibody

TGFB1I1 Antibody

CSB-PA050247
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTGFB1I1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TGFB1I1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7041
  • Target name
    TGFB1I1
  • Target description
    transforming growth factor beta 1 induced transcript 1
  • Target synonyms
    ARA55, HIC-5, HIC5, TSC-5, transforming growth factor beta-1-induced transcript 1 protein, androgen receptor coactivator 55 kDa protein, androgen receptor coactivator ARA55, androgen receptor-associated protein of 55 kDa, hydrogen peroxide-inducible clone 5 protein, hydrogen peroxide-inducible clone-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43294
  • Protein Name
    Transforming growth factor beta-1-induced transcript 1 protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HIC5/TGFB1I1 using anti-HIC5/TGFB1I1 antibody (A04630-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIC5/TGFB1I1 antigen affinity purified polyclonal antibody (Catalog # A04630-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIC5/TGFB1I1 at approximately 75 kDa. The expected band size for HIC5/TGFB1I1 is at 75 kDa.
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