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Western blot analysis of extracts of various cell lines using THPO Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using THPO Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using THPO Polyclonal Antibody at dilution of 1:1000.

THPO Polyclonal Antibody

E-AB-60463
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    THPO Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5 UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat kidney tissue lysate, Lane 5: rat thymus tissue lysate, Lane 6: rat PC-12 cell lysate, Lane 7: rat RH35 cell lysate, Lane 8: mouse SP20 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody (Catalog # A03222-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.
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