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Mouse splenocytes probed with TLR1 Polyclonal Antibody, Unconjugated (bs-1919R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with TLR1 Polyclonal Antibody, Unconjugated (bs-1919R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with TLR1 Polyclonal Antibody, Unconjugated (bs-1919R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

TLR1 Polyclonal Antibody

BS-1919R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Rat
TargetTLR1
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Overview

  • Supplier
    Bioss
  • Product Name
    TLR1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7096
  • Target name
    TLR1
  • Target description
    toll like receptor 1
  • Target synonyms
    CD281, TIL, TIL. LPRS5, rsc786, toll-like receptor 1, toll/interleukin-1 receptor-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15399
  • Protein Name
    Toll-like receptor 1
  • Reactivity
    Bovine, Canine, Equine, Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li C, Yu Y, Li W, et al. Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway. Sci Rep. 2017,7(1):5843. doi: 10.1038/s41598-017-06021-5
    Read this paper

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Figure 1. Western blot analysis of TLR1 using anti-TLR1 antibody (A00429-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody (Catalog # A00429-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD.
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