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FACS analysis of RAW264.7 cells using GTX29104 TLR4 antibody [MTS510]. Red : Primary antibody Green : isotype control Black : cells without antibody Antibody amount : 0.5μg
FACS analysis of RAW264.7 cells using GTX29104 TLR4 antibody [MTS510]. Red : Primary antibody Green : isotype control Black : cells without antibody Antibody amount : 0.5μg
FACS analysis of RAW264.7 cells using GTX29104 TLR4 antibody [MTS510]. Red : Primary antibody Green : isotype control Black : cells without antibody Antibody amount : 0.5μg

TLR4 antibody [MTS510]

GTX29104
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetTlr4
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Overview

  • Supplier
    GeneTex
  • Product Name
    TLR4 antibody [MTS510]
  • Delivery Days Customer
    9
  • Application Supplier Note
    FCM: 1 - 2 microg / 106 cells. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MTS510
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID21898
  • Target name
    Tlr4
  • Target description
    toll-like receptor 4
  • Target synonyms
    Lps, Ly87, Ran/M1, Rasl2-8, toll-like receptor 4, Toll like receptor 4, lipopolysaccharide response
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ9QUK6
  • Protein Name
    Toll-like receptor 4
  • Scientific Description
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Necrotic myocardial cells release damage-associated molecular patterns that provoke fibroblast activation in vitro and trigger myocardial inflammation and fibrosis in vivo. Zhang W et al., 2015 Jun 2, J Am Heart Assoc
    Read this paper

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Figure 1. Western blot analysis of Tlr4 using anti-Tlr4 antibody (A00017-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr4 antigen affinity purified polyclonal antibody (Catalog # A00017-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr4 at approximately 96 kDa. The expected band size for Tlr4 is at 96 kDa.
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