TNFSF11 / RANKL / TRANCE Antibody (Biotin)

LifeSpan BioSciences

TNFSF11 / RANKL / TRANCE Antibody (Biotin)

23

Human sRANK Ligand

ELISA: To detect hsRANKL by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. To detect hsRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sRANKL (LS-B7418) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA (0.25 - 1 µg/ml), WB (0.1 - 0.2 µg/ml) ELISA: To detect hsRANKL by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. To detect hsRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sRANKL (LS-B7418) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Western Blot, ELISA

Research Use Only

Polyclonal

Biotin

...

TNFSF11

TNF superfamily member 11

CD254; hRANKL2; ODF; OPGL; OPTB2; osteoclast differentiation factor; osteoprotegerin ligand; RANKL; receptor activator of nuclear factor kappa B ligand; sOdf; TNF-related activation-induced cytokine; TNLG6B; TRANCE; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11

Rabbit

Human

-20°C,2°C to 8°C,RT

12352203