TOP1 Antibody, HRP conjugated

Cusabio

TOP1 Antibody, HRP conjugated

20

ELISA

Research Use Only

Polyclonal

HRP

TOP1

DNA topoisomerase I

DNA topoisomerase 1; Scl-70 antigen; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase

Rabbit

IgG

DNA topoisomerase 1

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Human

-20°C or -80°C

12352203