Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [GTX31130] - Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (GTX31130). GTX31130 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT.
Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [GTX31130] - Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (GTX31130). GTX31130 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT.
TPH2 antibody
GTX31130
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetTPH2
Overview
- SupplierGeneTex
- Product NameTPH2 antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID121278
- Target nameTPH2
- Target descriptiontryptophan hydroxylase 2
- Target synonymsADHD7; neuronal tryptophan hydroxylase; NTPH; tryptophan 5-hydroxylase 2; tryptophan 5-monooxygenase 2
- HostRabbit
- IsotypeIgG
- Scientific DescriptionTryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
- ReactivityHuman, Mouse, Rabbit, Rat
- Storage Instruction2°C to 8°C
- UNSPSC12352203