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TPPP Antibody (YA2185)

HY-P82440
MedChem Express
TargetTPPP
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TPPP Antibody (YA2185)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID11076
  • Target name
    TPPP
  • Target description
    tubulin polymerization promoting protein
  • Target synonyms
    25 kDa brain-specific protein; brain specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; p24; p25; p25alpha; p25-alpha; TPPP/p25; TPPP1; tubulin polymerization-promoting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TPPP Antibody (YA2185) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2185), targeting TPPP, with a predicted molecular weight of 24 kDa (observed band size: 24 kDa). TPPP Antibody (YA2185) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TPPP using anti-TPPP antibody (A04389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPPP antigen affinity purified polyclonal antibody (Catalog # A04389-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPPP at approximately 24 kDa. The expected band size for TPPP is at 24 kDa.
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